Structural Characterization of the Complex between Hen Egg-White Lysozyme and Zr(IV) -Substituted Keggin Polyoxometalate as Artificial Protease.
Chemistry
; 21(33): 11692-5, 2015 Aug 10.
Article
em En
| MEDLINE
| ID: mdl-26179600
ABSTRACT
Successful co-crystallization of a noncovalent complex between hen egg-white lysozyme (HEWL) and the monomeric Zr(IV) -substituted Keggin polyoxometalate (POM) (Zr1 K1), (Et2 NH2)3 [Zr(PW11 O39)] (1), has been achieved, and its single-crystal X-ray structure has been determined. The dimeric Zr(IV) -substituted Keggin-type polyoxometalate (Zr1 K2), (Et2 NH2)10 [Zr(PW11 O39 )2] (2), has been previously shown to exhibit remarkable selectivity towards HEWL hydrolysis. The reported X-ray structure shows that the hydrolytically active Zr(IV) -substituted Keggin POM exists as a monomeric species. Prior to hydrolysis, this monomer interacts with HEWL in the vicinity of the previously identified cleavage sites found at Trp28-Val29 and Asn44-Arg45, through water-mediated H-bonding and electrostatic interactions. Three binding sites are observed at the interface of the negatively charged Keggin POM and the positively charged regions of HEWL at 1)â
Gly16, Tyr20, and Arg21; 2)â
Asn44, Arg45, and Asn46; and 3)â
Arg128.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Zircônio
/
Muramidase
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Compostos de Tungstênio
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article