Immunoproteomic Identification of p29 Antigen as the Elongation Factor-1α of Leishmania mexicana.
Vector Borne Zoonotic Dis
; 15(7): 449-52, 2015 Jul.
Article
em En
| MEDLINE
| ID: mdl-26186518
ABSTRACT
Previously, we identified five Leishmania mexicana antigens reacting with antibodies from cutaneous leishmaniasis patients, designated on the basis of their molecular weights as p26 (pI 7.8), p27 (pI 8.1), p28 (pI 8.6), p29 (pI 8.5), and p31 (pI 9.0). Among these antigens, p29 was most strongly recognized by the antibodies. Thereafter, p29 was identified as elongation factor-1α (EF-1α) of Leishmania mexicana through mass spectrometry analysis and western blot using a commercial antibody that reacted with EF-1α from different species. Our results showed that the p29 antigen of Leishmania mexicana is EF-1α.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Leishmania mexicana
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Leishmaniose Cutânea
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Fator 1 de Elongação de Peptídeos
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Antígenos de Protozoários
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
País como assunto:
Mexico
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article