PTEN mediates Notch-dependent stalk cell arrest in angiogenesis.
Nat Commun
; 6: 7935, 2015 Jul 31.
Article
em En
| MEDLINE
| ID: mdl-26228240
ABSTRACT
Coordinated activity of VEGF and Notch signals guides the endothelial cell (EC) specification into tip and stalk cells during angiogenesis. Notch activation in stalk cells leads to proliferation arrest via an unknown mechanism. By using gain- and loss-of-function gene-targeting approaches, here we show that PTEN is crucial for blocking stalk cell proliferation downstream of Notch, and this is critical for mouse vessel development. Endothelial deletion of PTEN results in vascular hyperplasia due to a failure to mediate Notch-induced proliferation arrest. Conversely, overexpression of PTEN reduces vascular density and abrogates the increase in EC proliferation induced by Notch blockade. PTEN is a lipid/protein phosphatase that also has nuclear phosphatase-independent functions. We show that both the catalytic and non-catalytic APC/C-Fzr1/Cdh1-mediated activities of PTEN are required for stalk cells' proliferative arrest. These findings define a Notch-PTEN signalling axis as an orchestrator of vessel density and implicate the PTEN-APC/C-Fzr1/Cdh1 hub in angiogenesis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
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Neovascularização Fisiológica
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Células Endoteliais
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Proliferação de Células
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PTEN Fosfo-Hidrolase
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Receptores Notch
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Ciclossomo-Complexo Promotor de Anáfase
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Proteínas Cdh1
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article