Structure elucidation of a new isoflavone by exclusive use of ¹H NMR measurements.
Magn Reson Chem
; 53(10): 860-5, 2015 Oct.
Article
em En
| MEDLINE
| ID: mdl-26255633
ABSTRACT
The leaves of Piscidia carthagenensis provided new 7,2',5'-trimethoxy-3',4'-methylenedioxyisoflavone (1), admixed with known 6,7-dimethoxy-3',4'-methylenedioxyisoflavone (2), and 5,4'-dihydroxy-7,2',5'-trimethoxyisoflavone (3), which were separated by extensive fractional solubillization. Selective irradiation of the H-5 "singlet" of 2 allowed distinction of the two methoxy group signals, whose chemical shift difference is only 0.004 ppm (1.2 Hz at 300 MHz). The (1)H and (13)C NMR data of 3 were assigned with the aid of HETCOR and gHMBC measurements. Although 1 looked inhomogeneous in the solid state, its solution structure followed from (1)H NMR measurements, where it looked homogeneous. To clarify the solid state aspect and confirm the structure of 1, two types of crystals were mechanically separated and subjected to single crystal X-ray diffraction measurements. This study revealed polymorphism because of the concomitant presence of orthorhombic and triclinic crystals, but showed no atropisomerism. The structure of 3 was also verified by X-ray diffraction crystallography.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Espectroscopia de Ressonância Magnética
/
Isoflavonas
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article