Continuous directed evolution of DNA-binding proteins to improve TALEN specificity.
Nat Methods
; 12(10): 939-42, 2015 Oct.
Article
em En
| MEDLINE
| ID: mdl-26258293
ABSTRACT
Nucleases containing programmable DNA-binding domains can alter the genomes of model organisms and have the potential to become human therapeutics. Here we present DNA-binding phage-assisted continuous evolution (DB-PACE) as a general approach for the laboratory evolution of DNA-binding activity and specificity. We used this system to generate transcription activator-like effectors nucleases (TALENs) with broadly improved DNA cleavage specificity, establishing DB-PACE as a versatile approach for improving the accuracy of genome-editing agents.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Evolução Molecular Direcionada
/
Desoxirribonucleases
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Proteínas de Ligação a DNA
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article