Schistosoma japonicum protein SjP40 inhibits TGF-ß1-induced activation of hepatic stellate cells.
Parasitol Res
; 114(11): 4251-7, 2015 Nov.
Article
em En
| MEDLINE
| ID: mdl-26268567
ABSTRACT
SjP40 is a major egg antigen of Schistosoma japonicum. In the present study, the authors investigated the effect of SjP40 in vitro on transforming growth factor-ß1 (TGF-ß1)- stimulated hepatic stellate cells (HSCs). LX-2, an immortalized human HSC line, was treated with purified recombinant SjP40 (rSjP40) in the presence or absence of TGF-ß1. Quantitative real-time polymerase chain reaction and western blot analysis were performed to determine messenger ribonucleic acid and protein of fibrogenic genes and TGF-ß signaling pathway. The results showed that expression of fibrogenic genes was significantly reduced by rSjP40. Furthermore, rSjP40 also suppressed the TGF-ß1-induced upregulation of Smads and ERK proteins. We also found that the effect of rSjP40 on HSCs was similar to SB431542, an inhibitor of type I TGF-ß receptor. In conclusion, the data suggest that SjP40 attenuates HSC activation, which might be, at least in part, mediated by inhibiting the TGF-ß and ERK signaling pathways.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Schistosoma japonicum
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Esquistossomose Japônica
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Proteínas de Helminto
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Fator de Crescimento Transformador beta1
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Células Estreladas do Fígado
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Interações Hospedeiro-Parasita
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article