Your browser doesn't support javascript.
loading
A Suite of Engineered GFP Molecules for Oligomeric Scaffolding.
Leibly, David J; Arbing, Mark A; Pashkov, Inna; DeVore, Natasha; Waldo, Geoffrey S; Terwilliger, Thomas C; Yeates, Todd O.
Afiliação
  • Leibly DJ; Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA; UCLA-DOE Institute of Genomics and Proteomics, University of California, Los Angeles, CA 90095, USA.
  • Arbing MA; UCLA-DOE Institute of Genomics and Proteomics, University of California, Los Angeles, CA 90095, USA.
  • Pashkov I; UCLA-DOE Institute of Genomics and Proteomics, University of California, Los Angeles, CA 90095, USA.
  • DeVore N; Bioscience Division, Los Alamos National Laboratory, MS M888, Los Alamos, NM 87545, USA.
  • Waldo GS; Bioscience Division, Los Alamos National Laboratory, MS M888, Los Alamos, NM 87545, USA.
  • Terwilliger TC; Bioscience Division, Los Alamos National Laboratory, MS M888, Los Alamos, NM 87545, USA.
  • Yeates TO; Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA; UCLA-DOE Institute of Genomics and Proteomics, University of California, Los Angeles, CA 90095, USA. Electronic address: yeates@mbi.ucla.edu.
Structure ; 23(9): 1754-1768, 2015 Sep 01.
Article em En | MEDLINE | ID: mdl-26278175
ABSTRACT
Applications ranging from synthetic biology to protein crystallization could be advanced by facile systems for connecting multiple proteins together in predefined spatial relationships. One approach to this goal is to engineer many distinct assembly forms of a single carrier protein or scaffold, to which other proteins of interest can then be readily attached. In this work we chose GFP as a scaffold and engineered many alternative oligomeric forms, driven by either specific disulfide bond formation or metal ion addition. We generated a wide range of spatial arrangements of GFP subunits from 11 different oligomeric variants, and determined their X-ray structures in a total of 33 distinct crystal forms. Some of the oligomeric GFP variants show geometric polymorphism depending on conditions, while others show considerable geometric rigidity. Potential future applications of this system are discussed.
Assuntos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Mutagênese Sítio-Dirigida / Proteínas de Fluorescência Verde Idioma: En Ano de publicação: 2015 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Mutagênese Sítio-Dirigida / Proteínas de Fluorescência Verde Idioma: En Ano de publicação: 2015 Tipo de documento: Article