PDGFRß and oncogenic mutant PDGFRα D842V promote disassembly of primary cilia through a PLCγ- and AURKA-dependent mechanism.
J Cell Sci
; 128(19): 3543-9, 2015 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-26290382
ABSTRACT
Primary cilia are microtubule-based sensory organelles projecting from most quiescent mammalian cells, which disassemble in cells cultured in serum-deprived conditions upon re-addition of serum or growth factors. Platelet-derived growth factors (PDGF) are implicated in deciliation, but the specific receptor isoforms and mechanisms involved are unclear. We report that PDGFRß promotes deciliation in cultured cells and provide evidence implicating PLCγ and intracellular Ca(2+) release in this process. Activation of wild-type PDGFRα alone did not elicit deciliation. However, expression of constitutively active PDGFRα D842V mutant receptor, which potently activates PLCγ (also known as PLCG1), caused significant deciliation, and this phenotype was rescued by inhibiting PDGFRα D842V kinase activity or AURKA. We propose that PDGFRß and PDGFRα D842V promote deciliation through PLCγ-mediated Ca(2+) release from intracellular stores, causing activation of calmodulin and AURKA-triggered deciliation.
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Cílios
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Receptor alfa de Fator de Crescimento Derivado de Plaquetas
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Receptor beta de Fator de Crescimento Derivado de Plaquetas
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Fosfolipase C gama
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Aurora Quinase A
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Animals
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En
Ano de publicação:
2015
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Article