Non-Equilibrium Isomer Distribution of the Gas-Phase Photoactive Yellow Protein Chromophore.
J Phys Chem Lett
; 3(16): 2259-63, 2012 Aug 16.
Article
em En
| MEDLINE
| ID: mdl-26295780
ABSTRACT
The conjugate base of para-coumaric acid, which can be conveniently generated in the gas phase by electrospray ionization (ESI), is a commonly used model system for the chromophore of the photoactive yellow protein. Here we report its gas-phase IR spectrum, which shows that the anion easily adopts a carboxylate structure lying 60 kJ/mol higher in energy than the global minimum phenoxide structure. Generation of the biologically more relevant phenoxide isomer by ESI can be achieved using dry acetonitrile as solvent.
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MEDLINE
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En
Ano de publicação:
2012
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Article