Functional footprinting of regulatory DNA.
Nat Methods
; 12(10): 927-30, 2015 Oct.
Article
em En
| MEDLINE
| ID: mdl-26322838
ABSTRACT
Regulatory regions harbor multiple transcription factor (TF) recognition sites; however, the contribution of individual sites to regulatory function remains challenging to define. We describe an approach that exploits the error-prone nature of genome editing-induced double-strand break repair to map functional elements within regulatory DNA at nucleotide resolution. We demonstrate the approach on a human erythroid enhancer, revealing single TF recognition sites that gate the majority of downstream regulatory function.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas Nucleares
/
Proteínas de Transporte
/
Sequências Reguladoras de Ácido Nucleico
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Pegada de DNA
/
Genômica
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article