A short-type peptidoglycan recognition protein from tongue sole (Cynoglossus semilaevis) promotes phagocytosis and defense against bacterial infection.
Fish Shellfish Immunol
; 47(1): 313-20, 2015 Nov.
Article
em En
| MEDLINE
| ID: mdl-26364742
ABSTRACT
Peptidoglycan recognition proteins (PGRPs) are members of the innate immune system that interact with bacteria by binding to bacterial peptidoglycan. In this study, we examined the expression and function of a short type of PGRP, CsPGRP-SC2, from tongue sole (Cynoglossus semilaevis). CsPGRP-SC2 contains 164 amino acid residues and shares 54.5%-65.3% overall sequence identities with other teleost PGRPs. CsPGRP-SC2 possesses an amidase domain with a conserved zinc binding site. CsPGRP-SC2 expression occurred in multiple tissues and was upregulated by bacterial and viral infection. Purified recombinant CsPGRP-SC2 (rCsPGRP-SC2) was able to bind and agglutinate Gram-positive bacteria in a Zn(2+)-dependent manner. rCsPGRP-SC2 enhanced the uptake of the bound bacteria by host phagocytes and reduced bacterial dissemination and colonization in host tissues. These results indicate that CsPGRP-SC2 is an innate immune factor that participates in host defense against bacterial infection.
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MEDLINE
Assunto principal:
Infecções Estreptocócicas
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Linguados
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Proteínas de Transporte
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Proteínas de Peixes
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Infecções por Vírus de DNA
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Doenças dos Peixes
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Imunidade Inata
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article