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Real-time reverse transcriptase polymerase chain reaction assays for Middle East Respiratory Syndrome.
Douglas, Christina E; Kulesh, David A; Jaissle, James G; Minogue, Timothy D.
Afiliação
  • Douglas CE; Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Disease, Fort Detrick, Maryland, USA.
  • Kulesh DA; Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Disease, Fort Detrick, Maryland, USA.
  • Jaissle JG; Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Disease, Fort Detrick, Maryland, USA.
  • Minogue TD; Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Disease, Fort Detrick, Maryland, USA. Electronic address: timothy.d.minogue.civ@mail.mil.
Mol Cell Probes ; 29(6): 511-513, 2015 Dec.
Article em En | MEDLINE | ID: mdl-26365228
ABSTRACT
Here we designed and tested two highly specific quantitative TaqMan(®)-MGB-based reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) assays for Middle East Respiratory Syndrome (MERS). The primers and probes for these assays were evaluated and found to have a limit of detection (LOD) of 0.005 plaque-forming units/PCR (pfu/PCR).
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Infecções por Coronavirus / Coronavirus / Reação em Cadeia da Polimerase Via Transcriptase Reversa Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Ano de publicação: 2015 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Infecções por Coronavirus / Coronavirus / Reação em Cadeia da Polimerase Via Transcriptase Reversa Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Ano de publicação: 2015 Tipo de documento: Article