Your browser doesn't support javascript.
loading
Spatial and temporal coordination of insulin granule exocytosis in intact human pancreatic islets.
Almaça, Joana; Liang, Tao; Gaisano, Herbert Y; Nam, Hong Gil; Berggren, Per-Olof; Caicedo, Alejandro.
Afiliação
  • Almaça J; Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, University of Miami Miller School of Medicine, 1580 NW 10th Ave, Miami, FL, 33136, USA. jalmaca@med.miami.edu.
  • Liang T; Diabetes Research Institute, University of Miami Miller School of Medicine, 1450 NW 10th Ave, Miami, FL, 33136, USA. jalmaca@med.miami.edu.
  • Gaisano HY; Center for Plant Aging Research, Institute for Basic Science and Department of New Biology, DGIST, Daegu, Republic of Korea. jalmaca@med.miami.edu.
  • Nam HG; Department of Medicine, University of Toronto, Toronto, ON, Canada.
  • Berggren PO; Department of Medicine, University of Toronto, Toronto, ON, Canada.
  • Caicedo A; Center for Plant Aging Research, Institute for Basic Science and Department of New Biology, DGIST, Daegu, Republic of Korea.
Diabetologia ; 58(12): 2810-8, 2015 Dec.
Article em En | MEDLINE | ID: mdl-26376795
ABSTRACT
AIMS/

HYPOTHESIS:

Insulin secretion is widely studied because it plays a central role in glucose homeostasis and diabetes. Processes from insulin granule fusion in beta cells to in vivo insulin secretion have been elucidated, but data at the cellular level do not fully account for several aspects of the macroscopic secretory pattern. Here we investigated how individual secretory events are coordinated spatially and temporally within intact human islets.

METHODS:

We used the fluorescent probe neuropeptide Y (NPY)-pHluorin to visualise insulin granule secretion in isolated intact human islets.

RESULTS:

We found that individual beta cells respond to increases in glucose concentration by releasing insulin granules in very discrete bursts with periods consistent with in vivo pulsatile insulin secretion. In successive secretory bursts during prolonged exposure to high glucose levels, secretory events progressively localised to preferential release sites, coinciding with the transition to second phase insulin secretion. Granule secretion was very synchronised in neighbouring beta cells, forming discrete regional clusters of activity. CONCLUSIONS/

INTERPRETATION:

These results reveal how individual secretory events are coordinated to produce pulsatile insulin secretion from human islets.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ilhotas Pancreáticas / Exocitose Limite: Adult / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ilhotas Pancreáticas / Exocitose Limite: Adult / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2015 Tipo de documento: Article