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Prevalence of Transmitted Drug Resistance Mutations in HIV-1-Infected Drug-Naive Patients from Urban and Suburban Regions of Kenya.
Onsongo, Simon; Abidi, Syed Hani; Khamadi, Samoel; Shah, Reena; Kageha, Sheila; Ojwang, Peter; Ali, Syed; Okinda, Nancy.
Afiliação
  • Onsongo S; 1 Aga Khan University Hospital , Nairobi, Kenya .
  • Abidi SH; 2 Department of Biological and Biomedical Sciences, Aga Khan University , Karachi, Pakistan .
  • Khamadi S; 3 U.S. Military HIV Research Program, Henry M. Jackson Foundation Medical Research International , Mbeya, Tanzania .
  • Shah R; 1 Aga Khan University Hospital , Nairobi, Kenya .
  • Kageha S; 4 Kenya Medical Research Institute , Nairobi, Kenya .
  • Ojwang P; 1 Aga Khan University Hospital , Nairobi, Kenya .
  • Ali S; 5 Department of Biomedical Sciences, Nazarbayer School of Medicine, Nazarbayer University, Astana, Kazakstan.
  • Okinda N; 1 Aga Khan University Hospital , Nairobi, Kenya .
AIDS Res Hum Retroviruses ; 32(3): 220-5, 2016 Mar.
Article em En | MEDLINE | ID: mdl-26401720
HIV was first described in Kenya in 1984-1985. Currently, Kenya has an estimated HIV-1 prevalence of 6.2%. With the introduction of antiretroviral drugs, the survival of most HIV patients has been prolonged markedly. However, this is greatly threatened by increasing rates of antiretroviral dug resistance, which may eventually lead to suboptimal treatment outcomes. The objective of this study was to characterize currently occurring antiretroviral drug resistance mutations among drug-naive patients visiting two referral hospitals in Kenya. Using polymerase chain reaction, the HIV protease gene was amplified from blood samples of 63 study participants. The sequences were used to determine HIV-1 subtype and presence/prevalence of mutations associated with resistance to protease inhibitors. Finally, the protease gene was variably measured using Shannon entropy analysis. Analysis of frequency of HIV-1 subtypes revealed subtype A to be the predominant subtype, while the analysis of drug resistance mutations revealed the presence of four minor drug resistance mutations associated weakly with resistance to protease inhibitors. Among these mutations, L33I was the most prevalent mutation. Shannon entropy analysis revealed high genomic variability, especially in region spanning nucleotides 1-55, 113-170, and 205-240. This study warrants the need for dedicated efforts to improve compliance to antiretroviral therapy and reduce transmitted resistance rates, which will greatly ensure the therapeutic efficacy of antiretroviral drugs.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Infecções por HIV / HIV-1 / Mutação de Sentido Incorreto / Farmacorresistência Viral / Genótipo Tipo de estudo: Observational_studies / Prevalence_studies / Risk_factors_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged País como assunto: Africa Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Infecções por HIV / HIV-1 / Mutação de Sentido Incorreto / Farmacorresistência Viral / Genótipo Tipo de estudo: Observational_studies / Prevalence_studies / Risk_factors_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged País como assunto: Africa Idioma: En Ano de publicação: 2016 Tipo de documento: Article