Synthesis and solid state NMR characterization of novel peptide/silica hybrid materials.
Solid State Nucl Magn Reson
; 72: 73-8, 2015 Nov.
Article
em En
| MEDLINE
| ID: mdl-26411982
ABSTRACT
The successful synthesis and solid state NMR characterization of silica-based organic-inorganic hybrid materials is presented. For this, collagen-like peptides are immobilized on carboxylate functionalized mesoporous silica (COOH/SiOx) materials. A pre-activation of the silica material with TSTU (O-(N-Succinimidyl)-N,N,N',N'-tetramethyluronium tetrafluoroborate) is performed to enable a covalent binding of the peptides to the linker. The success of the covalent immobilization is indicated by the decrease of the (13)C CP-MAS NMR signal of the TSTU moiety. A qualitative distinction between covalently bound and adsorbed peptide is feasible by (15)N CP-MAS Dynamic Nuclear Polarization (DNP). The low-field shift of the (15)N signal of the peptide's N-terminus clearly identifies it as the binding site. The DNP enhancement allows the probing of natural abundance (15)N nuclei, rendering expensive labeling of peptides unnecessary.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oligopeptídeos
/
Dióxido de Silício
Tipo de estudo:
Qualitative_research
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article