[Drug resistance of colon cancer cells to 5-fluorouracil mediated by microRNA-21].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
; 32(5): 620-4, 2015 Oct.
Article
em Zh
| MEDLINE
| ID: mdl-26418978
ABSTRACT
OBJECTIVE To explore downstream regulatory pathway of microRNA-21 (miR-21) in colon cancer cells (RKO) through detecting miR-21 and its target PDCD4, and the influence of miR-21 regulation on the sensitivity of RKO cells to 5-fluorouracil (5-FU). METHODS 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the effect of 5-FU on the viability of RKO cells with knockout of miR-21 or high expression of PDCD4. Real-time was used to determine the expression of PDCD4, ABCC5 and CD44 in RKO cell after knockout of miR-21. RESULTS MTT assay reveals that the IC50 of 5-FU in RKO-WT cells (52.82 ± 0.06 umol/L) was about 67% higher than in miR-21 knockout cells (32.23 ± 0.05 umol/L) (P < 0.05), and the apoptosis ratio elevated after knockout of miR-21. High expression of PDCD4, a target gene of miR-21, can negatively regulate the expression of ABC transporter ABCC5 and the stem cell marker CD44. CONCLUSION MiR-21 can mediate the drug resistance to 5-FU by inhibiting its target PDCD4, which can regulate the expression of ABCC5 and CD44 genes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Neoplasias do Colo
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Resistencia a Medicamentos Antineoplásicos
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MicroRNAs
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Fluoruracila
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Antimetabólitos Antineoplásicos
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2015
Tipo de documento:
Article