Optofluidic wavelength division multiplexing for single-virus detection.
Proc Natl Acad Sci U S A
; 112(42): 12933-7, 2015 Oct 20.
Article
em En
| MEDLINE
| ID: mdl-26438840
ABSTRACT
Optical waveguides simultaneously transport light at different colors, forming the basis of fiber-optic telecommunication networks that shuttle data in dozens of spectrally separated channels. Here, we reimagine this wavelength division multiplexing (WDM) paradigm in a novel context--the differentiated detection and identification of single influenza viruses on a chip. We use a single multimode interference (MMI) waveguide to create wavelength-dependent spot patterns across the entire visible spectrum and enable multiplexed single biomolecule detection on an optofluidic chip. Each target is identified by its time-dependent fluorescence signal without the need for spectral demultiplexing upon detection. We demonstrate detection of individual fluorescently labeled virus particles of three influenza A subtypes in two implementations labeling of each virus using three different colors and two-color combinatorial labeling. By extending combinatorial multiplexing to three or more colors, MMI-based WDM provides the multiplexing power required for differentiated clinical tests and the growing field of personalized medicine.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vírus da Influenza A
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Técnicas Analíticas Microfluídicas
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Dispositivos Ópticos
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article