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Intermolecular Paramagnetic Relaxation Enhancement (PRE) Studies of Transient Complexes in Intrinsically Disordered Proteins.
Janowska, Maria K; Baum, Jean.
Afiliação
  • Janowska MK; Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, NJ, 08854, USA.
  • Baum J; Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, NJ, 08854, USA. jean.baum@rutgers.edu.
Methods Mol Biol ; 1345: 45-53, 2016.
Article em En | MEDLINE | ID: mdl-26453204
NMR interchain paramagnetic relaxation enhancement (PRE) techniques are a very powerful approach for detecting transient interchain interactions between intrinsically disordered proteins. These experiments, requiring a mixed sample containing a 1:1 ratio of isotope-labeled (15)N protein and natural abundance (14)N protein with a paramagnetic spin label, provide data that is limited to interchain interactions only. Application of these experiments to weakly associated transient species such as those that are present in the very early stages of self-assembly processes will aid our understanding of protein aggregation or fibril formation processes.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espectroscopia de Ressonância de Spin Eletrônica / Ressonância Magnética Nuclear Biomolecular / Proteínas Intrinsicamente Desordenadas Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espectroscopia de Ressonância de Spin Eletrônica / Ressonância Magnética Nuclear Biomolecular / Proteínas Intrinsicamente Desordenadas Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article