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[A Large Number of Circulating Tumor Cells(CTCs)Can Be Isolated from Samples Obtained by Using Leukapheresis Procedures].
Soya, Ryoko; Taguchi, Jyunichi; Nagakawa, Yuichi; Takahashi, Osamu; Sandoh, Norimasa; Hosokawa, Yuichi; Kasuya, Kazuhiko; Umeda, Naoki; Okamoto, Masato; Tsujitani, Shunichi; Tsuchida, Akihiko.
Afiliação
  • Soya R; Dept. of Gastrointestinal Pediatric Surgery, Tokyo Medical University.
Gan To Kagaku Ryoho ; 42(9): 1069-72, 2015 Sep.
Article em Ja | MEDLINE | ID: mdl-26469161
ABSTRACT
We hypothesized that a large number of circulating tumor cells(CTCs)may be isolated from samples obtained by using the leukapheresis procedures that are utilized to collect peripheral blood mononuclear cells for dendritic cell vaccine therapy. We utilized the CellSearch System to determine the number of CTCs in samples obtained by using leukapheresis in 7 patients with colorectal cancer, 5 patients with breast cancer, and 3 patients with gastric cancer. In all patients, a large number of CTCs were isolated. The mean number of CTCs per tumor was 17.1(range 10-34)in colorectal cancer, 10.0(range 2-27)in breast cancer, and 24.0(range 2-42)in gastric cancer. We succeeded in culturing the isolated CTCs from 7 patients with colorectal cancer, 5 patients with breast cancer, and 3 patients with gastric cancer. In conclusion, compared to conventional methods, a large number of CTCs can be obtained by using leukapheresis procedures. The molecular analyses of the CTCs isolated by using this method should be promising in the development of personalized cancer treatments.
Assuntos
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Base de dados: MEDLINE Assunto principal: Separação Celular / Leucaférese / Células Neoplásicas Circulantes Limite: Aged / Female / Humans / Male / Middle aged Idioma: Ja Ano de publicação: 2015 Tipo de documento: Article
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Base de dados: MEDLINE Assunto principal: Separação Celular / Leucaférese / Células Neoplásicas Circulantes Limite: Aged / Female / Humans / Male / Middle aged Idioma: Ja Ano de publicação: 2015 Tipo de documento: Article