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Alcohol-dysregulated miR-30a and miR-934 in head and neck squamous cell carcinoma.
Saad, Maarouf A; Kuo, Selena Z; Rahimy, Elham; Zou, Angela E; Korrapati, Avinaash; Rahimy, Mehran; Kim, Elizabeth; Zheng, Hao; Yu, Michael Andrew; Wang-Rodriguez, Jessica; Ongkeko, Weg M.
Afiliação
  • Saad MA; Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of California, La Jolla, San Diego, CA, USA. masaad@ucsd.edu.
  • Kuo SZ; Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of California, La Jolla, San Diego, CA, USA. szkuo@ucsd.edu.
  • Rahimy E; Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of California, La Jolla, San Diego, CA, USA. elham.rahimy@yale.edu.
  • Zou AE; Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of California, La Jolla, San Diego, CA, USA. azou@ucsd.edu.
  • Korrapati A; Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of California, La Jolla, San Diego, CA, USA. akorrapa@ucsd.edu.
  • Rahimy M; Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of California, La Jolla, San Diego, CA, USA. mrahimy@ucsd.edu.
  • Kim E; Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of California, La Jolla, San Diego, CA, USA. elkim@ucsd.edu.
  • Zheng H; Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of California, La Jolla, San Diego, CA, USA. h3zheng@ucsd.edu.
  • Yu MA; Division of Otolaryngology-Head and Neck Surgery, Department of Surgery, University of California, La Jolla, San Diego, CA, USA. michael.yu@my.rfums.org.
  • Wang-Rodriguez J; Department of Pathology, Veterans Administration Health Care System, San Diego, CA, USA. jessica.wang-rodriguez@va.gov.
  • Ongkeko WM; Department of Pathology, University of California, San Diego, CA, USA. jessica.wang-rodriguez@va.gov.
Mol Cancer ; 14: 181, 2015 Oct 15.
Article em En | MEDLINE | ID: mdl-26472042
BACKGROUND: Alcohol consumption is a well-established risk factor for head and neck squamous cell carcinoma (HNSCC); however, the molecular mechanisms by which alcohol promotes HNSCC pathogenesis and progression remain poorly understood. Our study sought to identify microRNAs that are dysregulated in alcohol-associated HNSCC and investigate their contribution to the malignant phenotype. METHOD: Using RNA-sequencing data from 136 HNSCC patients, we compared the expression levels of 1,046 microRNAs between drinking and non-drinking cohorts. Dysregulated microRNAs were verified by qRT-PCR in normal oral keratinocytes treated with biologically relevant doses of ethanol and acetaldehyde. The most promising microRNA candidates were investigated for their effects on cellular proliferation and invasion, sensitivity to cisplatin, and expression of cancer stem cell genes. Finally, putative target genes were identified and evaluated in vitro to further establish roles for these miRNAs in alcohol-associated HNSCC. RESULTS: From RNA-sequencing analysis we identified 8 miRNAs to be significantly upregulated in alcohol-associated HNSCCs. qRT-PCR experiments determined that among these candidates, miR-30a and miR-934 were the most highly upregulated in vitro by alcohol and acetaldehyde. Overexpression of miR-30a and miR-934 in normal and HNSCC cell lines produced up to a 2-fold increase in cellular proliferation, as well as induction of the anti-apoptotic gene BCL-2. Upon inhibition of these miRNAs, HNSCC cell lines exhibited increased sensitivity to cisplatin and reduced matrigel invasion. miRNA knockdown also indicated direct targeting of several tumor suppressor genes by miR-30a and miR-934. CONCLUSIONS: Alcohol induces the dysregulation of miR-30a and miR-934, which may play crucial roles in HNSCC pathogenesis and progression. Future investigation of the alcohol-mediated pathways effecting these transformations will prove valuable for furthering the understanding and treatment of alcohol-associated HNSCC.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Carcinoma de Células Escamosas / Regulação Neoplásica da Expressão Gênica / MicroRNAs / Álcoois / Neoplasias de Cabeça e Pescoço Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Adult / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Carcinoma de Células Escamosas / Regulação Neoplásica da Expressão Gênica / MicroRNAs / Álcoois / Neoplasias de Cabeça e Pescoço Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Adult / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2015 Tipo de documento: Article