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Effects of Transcription Elongation Rate and Xrn2 Exonuclease Activity on RNA Polymerase II Termination Suggest Widespread Kinetic Competition.
Fong, Nova; Brannan, Kristopher; Erickson, Benjamin; Kim, Hyunmin; Cortazar, Michael A; Sheridan, Ryan M; Nguyen, Tram; Karp, Shai; Bentley, David L.
Afiliação
  • Fong N; Deptartment of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, PO Box 6511, Aurora, CO 80045, USA.
  • Brannan K; Deptartment of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, PO Box 6511, Aurora, CO 80045, USA.
  • Erickson B; Deptartment of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, PO Box 6511, Aurora, CO 80045, USA.
  • Kim H; Deptartment of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, PO Box 6511, Aurora, CO 80045, USA.
  • Cortazar MA; Deptartment of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, PO Box 6511, Aurora, CO 80045, USA.
  • Sheridan RM; Deptartment of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, PO Box 6511, Aurora, CO 80045, USA.
  • Nguyen T; Deptartment of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, PO Box 6511, Aurora, CO 80045, USA.
  • Karp S; Deptartment of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, PO Box 6511, Aurora, CO 80045, USA.
  • Bentley DL; Deptartment of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, PO Box 6511, Aurora, CO 80045, USA. Electronic address: david.bentley@ucdenver.edu.
Mol Cell ; 60(2): 256-67, 2015 Oct 15.
Article em En | MEDLINE | ID: mdl-26474067
ABSTRACT
The torpedo model of transcription termination asserts that the exonuclease Xrn2 attacks the 5'PO4-end exposed by nascent RNA cleavage and chases down the RNA polymerase. We tested this mechanism using a dominant-negative human Xrn2 mutant and found that it delayed termination genome-wide. Xrn2 nuclease inactivation caused strong termination defects downstream of most poly(A) sites and modest delays at some histone and U snRNA genes, suggesting that the torpedo mechanism is not limited to poly(A) site-dependent termination. A central untested feature of the torpedo model is that there is kinetic competition between the exonuclease and the pol II elongation complex. Using pol II rate mutants, we found that slow transcription robustly shifts termination upstream, and fast elongation extends the zone of termination further downstream. These results suggest that kinetic competition between elongating pol II and the Xrn2 exonuclease is integral to termination of transcription on most human genes.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Poli A / RNA Polimerase II / RNA Mensageiro / Exorribonucleases / Elongação da Transcrição Genética / Terminação da Transcrição Genética Limite: Humans Idioma: En Ano de publicação: 2015 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Poli A / RNA Polimerase II / RNA Mensageiro / Exorribonucleases / Elongação da Transcrição Genética / Terminação da Transcrição Genética Limite: Humans Idioma: En Ano de publicação: 2015 Tipo de documento: Article