A ku70 null mutant improves gene targeting frequency in the fungal pathogen Verticillium dahliae.
World J Microbiol Biotechnol
; 31(12): 1889-97, 2015 Dec.
Article
em En
| MEDLINE
| ID: mdl-26475327
To overcome the challenges met with gene deletion in the plant pathogen Verticillium dahliae, a mutant strain with impaired non-homologous end joining DNA repair was generated to improve targeted gene replacement frequencies. A V. dahliae 991 ΔVdku70 null mutant strain was generated using Agrobacterium tumefaciens-mediated transformation. Despite having impaired non-homologous end joining DNA repair function, the ΔVdku70 strain exhibited normal growth, reproduction capability, and pathogenicity when compared with the wild-type strain. When the ΔVdku70 strain was used to delete 2-oxoglutarate dehydrogenase E2, ferric reductase transmembrane component 3 precursor, and ferric reductase transmembrane component 6 genes, gene replacement frequencies ranged between 22.8 and 34.7% compared with 0.3 and 0.5 % in the wild-type strain. The ΔVdku70 strain will be a valuable tool to generate deletion strains when studying factors that underlie virulence and pathogenesis in this filamentous fungus.
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Base de dados:
MEDLINE
Assunto principal:
Virulência
/
Marcação de Genes
/
Verticillium
/
Antígenos Nucleares
/
Proteínas de Ligação a DNA
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article