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Tissue matrix arrays for high-throughput screening and systems analysis of cell function.
Beachley, Vince Z; Wolf, Matthew T; Sadtler, Kaitlyn; Manda, Srikanth S; Jacobs, Heather; Blatchley, Michael R; Bader, Joel S; Pandey, Akhilesh; Pardoll, Drew; Elisseeff, Jennifer H.
Afiliação
  • Beachley VZ; Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland, USA.
  • Wolf MT; Department of Biomedical Engineering, Rowan University, Glassboro, New Jersey, USA.
  • Sadtler K; Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland, USA.
  • Manda SS; Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland, USA.
  • Jacobs H; McKusick-Nathans Institute of Genetic Medicine, Baltimore, Maryland, USA.
  • Blatchley MR; Institute of Bioinformatics, International Technology Park, Bangalore, India.
  • Bader JS; Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland, USA.
  • Pandey A; Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland, USA.
  • Pardoll D; High-Throughput Biology Center, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
  • Elisseeff JH; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland, USA.
Nat Methods ; 12(12): 1197-204, 2015 Dec.
Article em En | MEDLINE | ID: mdl-26480475
ABSTRACT
Cell and protein arrays have demonstrated remarkable utility in the high-throughput evaluation of biological responses; however, they lack the complexity of native tissue and organs. Here we spotted tissue extracellular matrix (ECM) particles as two-dimensional (2D) arrays or incorporated them with cells to generate three-dimensional (3D) cell-matrix microtissue arrays. We then investigated the responses of human stem, cancer and immune cells to tissue ECM arrays originating from 11 different tissues. We validated the 2D and 3D arrays as representative of the in vivo microenvironment by means of quantitative analysis of tissue-specific cellular responses, including matrix production, adhesion and proliferation, and morphological changes after culture. The biological outputs correlated with tissue proteomics, and network analysis identified several proteins linked to cell function. Our methodology enables broad screening of ECMs to connect tissue-specific composition with biological activity, providing a new resource for biomaterials research and further understanding of regeneration and disease mechanisms.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteoma / Proteômica / Matriz Extracelular / Ensaios de Triagem em Larga Escala Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Limite: Animals / Female / Humans Idioma: En Ano de publicação: 2015 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteoma / Proteômica / Matriz Extracelular / Ensaios de Triagem em Larga Escala Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Limite: Animals / Female / Humans Idioma: En Ano de publicação: 2015 Tipo de documento: Article