Low-Cost Peptide Microarrays for Mapping Continuous Antibody Epitopes.
Methods Mol Biol
; 1352: 67-83, 2016.
Article
em En
| MEDLINE
| ID: mdl-26490468
ABSTRACT
With the increasing need for understanding antibody specificity in antibody and vaccine research, pepscan assays provide a rapid method for mapping and profiling antibody responses to continuous epitopes. We have developed a relatively low-cost method to generate peptide microarray slides for studying antibody binding. Using a setup of an IntavisAG MultiPep RS peptide synthesizer, a Digilab MicroGrid II 600 microarray printer robot, and an InnoScan 1100 AL scanner, the method allows the interrogation of up to 1536 overlapping, alanine-scanning, and mutant peptides derived from the target antigens. Each peptide is tagged with a polyethylene glycol aminooxy terminus to improve peptide solubility, orientation, and conjugation efficiency to the slide surface.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Mapeamento de Epitopos
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Análise Serial de Proteínas
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Anticorpos
Tipo de estudo:
Health_economic_evaluation
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article