Determination of Apparent Amylose Content in Rice by Using Paper-Based Microfluidic Chips.
J Agric Food Chem
; 63(44): 9863-8, 2015 Nov 11.
Article
em En
| MEDLINE
| ID: mdl-26495809
ABSTRACT
Determination of apparent amylose content in rice is a key function for rice research and the rice industry. In this paper, a novel approach with paper-based microfluidic chip is reported to determine apparent amylose content in rice. The conventional color reaction between amylose and iodine was employed. Blue color of amylose-iodine complex generated on-chip was converted to gray and measured with Photoshop after the colored chip was scanned. The method for preparation of the paper chip is described. In situ generation of iodine for on-chip color reaction was designed, and factors influencing color reaction were investigated in detail. Elimination of yellow color interference of excess iodine by exploiting color removal function of Photoshop was presented. Under the optimized conditions, apparent amylose content in rice ranging from 1.5 to 26.4% can be determined, and precision was 6.3%. The analytical results obtained with the developed approach were in good agreement with those with the continuous flow analyzer method.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oryza
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Microfluídica
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Amilose
Tipo de estudo:
Evaluation_studies
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article