CRISPR/Cas9 genome editing technique and its application in site-directed genome modification of animals.
Yi Chuan
; 37(10): 1011-20, 2015 10.
Article
em En
| MEDLINE
| ID: mdl-26496753
ABSTRACT
CRISPR/Cas system, which uses CRISPR RNAs (crRNAs) to guide Cas nuclease to silence invading nucleic acids, is self-defense system against exogenous virus or plasmid in bacteria and archaea. Through molecular modification, the typeâ
¡CRISPR/Cas system has become a highly efficient site-directed genome editing technique, which is simpler than zinc-finger nucleases (ZFNs) and transcription activator like effector nucleases (TALENs) and easier to be designed and applied. In this review, we summarize the evolutionary history of CRISPR/Cas9 system, the working principle and modification process of type â
¡ CRISPR/Cas and its application in animal genome modification. We also analyze the existing problems and improvement program of the CRISPR/Cas9 system as well as its application prospect combined with successful cases, which may provide innovative perspectives on improving animal traits and establishing animal models of human diseases.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Engenharia Genética
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Mutagênese Sítio-Dirigida
/
Genoma
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Modelos Animais de Doenças
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Sistemas CRISPR-Cas
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article