Pore-forming toxins: Properties, diversity, and uses as tools to image sphingomyelin and ceramide phosphoethanolamine.
Biochim Biophys Acta
; 1858(3): 576-92, 2016 Mar.
Article
em En
| MEDLINE
| ID: mdl-26498396
ABSTRACT
Pore-forming toxins (PFTs) represent a unique class of highly specific lipid-binding proteins. The cytotoxicity of these compounds has been overcome through crystallographic structure and mutation studies, facilitating the development of non-toxic lipid probes. As a consequence, non-toxic PFTs have been utilized as highly specific probes to visualize the diversity and dynamics of lipid nanostructures in living and fixed cells. This review is focused on the application of PFTs and their non-toxic analogs as tools to visualize sphingomyelin and ceramide phosphoethanolamine, two major phosphosphingolipids in mammalian and insect cells, respectively. This article is part of a Special Issue entitled Pore-Forming Toxins edited by Mauro Dalla Serra and Franco Gambale.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Esfingomielinas
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Sondas Moleculares
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Proteínas Citotóxicas Formadoras de Poros
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Imagem Molecular
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article