Epitope-Specific Anti-hCG Vaccines on a Virus Like Particle Platform.
PLoS One
; 10(10): e0141407, 2015.
Article
em En
| MEDLINE
| ID: mdl-26516771
ABSTRACT
The possibility of a contraceptive vaccine targeting human chorionic gonadotropin has long been recognized, but never fully realized. Here we describe an epitope-specific approach based on immunogenic display of hCG-derived peptides on virus-like particles of RNA bacteriophage. A number of recombinant VLPs were constructed, each displaying a different hCG-derived peptide. Some were taken from the disordered C-terminal tail of the hormone, another came from an internal loop, and yet another was an epitope mimic produced by affinity-selection on an hCG-neutralizing antibody target. Immunization of mice with some VLPs yielded antisera that bound the hormone and neutralized hCG biological activity.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Levivirus
/
Vacinas Anticoncepcionais
/
Proteínas do Capsídeo
/
Vacinas de Partículas Semelhantes a Vírus
/
Gonadotropina Coriônica
/
Epitopos
Tipo de estudo:
Clinical_trials
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article