Macrophage activation induced by Brucella DNA suppresses bacterial intracellular replication via enhancing NO production.
Microb Pathog
; 89: 177-83, 2015 Dec.
Article
em En
| MEDLINE
| ID: mdl-26523973
ABSTRACT
Brucella DNA can be sensed by TLR9 on endosomal membrane and by cytosolic AIM2-inflammasome to induce proinflammatory cytokine production that contributes to partially activate innate immunity. Additionally, Brucella DNA has been identified to be able to act as a major bacterial component to induce type I IFN. However, the role of Brucella DNA in Brucella intracellular growth remains unknown. Here, we showed that stimulation with Brucella DNA promote macrophage activation in TLR9-dependent manner. Activated macrophages can suppresses wild type Brucella intracellular replication at early stage of infection via enhancing NO production. We also reported that activated macrophage promotes bactericidal function of macrophages infected with VirB-deficient Brucella at the early or late stage of infection. This study uncovers a novel function of Brucella DNA, which can help us further elucidate the mechanism of Brucella intracellular survival.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Brucella
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DNA Bacteriano
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Ativação de Macrófagos
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Macrófagos
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Óxido Nítrico
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article