Effects of 2-deoxyglucose on Saccharomyces cerevisiae as observed by in vivo 31P-NMR.
FEMS Microbiol Lett
; 48(1): 25-8, 1989 Jan 01.
Article
em En
| MEDLINE
| ID: mdl-2653955
ABSTRACT
Saccharomyces cerevisiae cells were treated with 2-deoxyglucose (1 mM) and the effects induced in the levels of phosphorus compounds and in the internal pH were monitored using 31P-NMR. Upon incubation with 2-deoxyglucose a strong decrease in the polyphosphate level was observed and the cytoplasmic pH decreased by about 0.4 units. This shows that 2-deoxyglucose strongly interferes with the cell conditions and consequently, the results of experiments in which 2-deoxyglucose was used to obtain deenergized cells should be carefully reanalysed.
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Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Desoxiaçúcares
/
Glucose-6-Fosfato
/
Desoxiglucose
Idioma:
En
Ano de publicação:
1989
Tipo de documento:
Article