Instructing Perisomatic Inhibition by Direct Lineage Reprogramming of Neocortical Projection Neurons.
Neuron
; 88(3): 475-83, 2015 Nov 04.
Article
em En
| MEDLINE
| ID: mdl-26539889
ABSTRACT
During development of the cerebral cortex, local GABAergic interneurons recognize and pair with excitatory projection neurons to ensure the fine excitatory-inhibitory balance essential for proper circuit function. Whether the class-specific identity of projection neurons has a role in the establishment of afferent inhibitory synapses is debated. Here, we report that direct in vivo lineage reprogramming of layer 2/3 (L2/3) callosal projection neurons (CPNs) into induced corticofugal projection neurons (iCFuPNs) increases inhibitory input onto the converted neurons to levels similar to that of endogenous CFuPNs normally found in layer 5 (L5). iCFuPNs recruit increased numbers of inhibitory perisomatic synapses from parvalbumin (PV)-positive interneurons, with single-cell precision and despite their ectopic location in L2/3. The data show that individual reprogrammed excitatory projection neurons extrinsically modulate afferent input by local PV(+) interneurons, suggesting that projection neuron class-specific identity can actively control the wiring of the cortical microcircuit.
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Base de dados:
MEDLINE
Assunto principal:
Neocórtex
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Corpo Caloso
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Rede Nervosa
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Inibição Neural
/
Neurônios
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article