Genome-Wide Analysis of Protein and mRNA Copy Numbers in Single Escherichia coli Cells with Single-Molecule Sensitivity.
Methods Mol Biol
; 1346: 55-67, 2015.
Article
em En
| MEDLINE
| ID: mdl-26542715
ABSTRACT
Single-cell proteomic and transcriptomic analysis is an emerging approach for providing quantitative and comprehensive characterization of gene functions in individual cells. This analysis, however, is often hampered by insufficient sensitivity for detecting low copy gene expression products such as transcription factors and regulators. Here I describe a method for the quantitative genome-wide analysis of single-cell protein and mRNA copy numbers with single molecule sensitivity for the model organism Escherichia coli.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
/
Dosagem de Genes
/
Perfilação da Expressão Gênica
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Proteínas de Escherichia coli
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Técnicas Analíticas Microfluídicas
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Escherichia coli
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article