Evaluation of an in vitro muscle contraction model in mouse primary cultured myotubes.
Anal Biochem
; 497: 36-8, 2016 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-26548957
ABSTRACT
To construct an in vitro contraction model with the primary cultured myotubes, we isolated satellite cells from the mouse extensor digitorum longus. Differentiated myotubes possessed a greater number of sarcomere assemblies and higher expression levels of myosin heavy chain, cytochrome c oxidase IV, and myoglobin than in C2C12 myotubes. In agreement with these results regarding the sarcomere assemblies and protein expressions, the primary myotubes showed higher contractile activity stimulated by the electric pulses than that in the C2C12 myotubes. These data suggest that mouse primary myotubes will be a valuable research tool as an in vitro muscle contraction model.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Músculo Esquelético
/
Fibras Musculares Esqueléticas
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Contração Muscular
Limite:
Animals
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article