Temporal and organ-specific detection of cNMPs including cUMP in the zebrafish.
Biochem Biophys Res Commun
; 468(4): 708-12, 2015 Dec 25.
Article
em En
| MEDLINE
| ID: mdl-26551461
The cyclic pyrimidine nucleotides cCMP and cUMP occur in mammalian cell lines. Recently, cCMP was also identified in mouse organs. Due to technical difficulties, it has not been possible to detect cUMP in organs or tissues yet. Here, we have generated a temporal profile of the occurrence of nucleoside 3',5'-cyclic monophosphates during different developmental stages of embryogenesis and in different organs of the adult zebrafish Danio rerio. Cyclic nucleotides were quantified by high performance liquid chromatography quadrupole tandem mass spectrometry. The identity of cCMP and cUMP in the zebrafish was confirmed by high performance liquid chromatography quadrupole time-of-flight mass spectrometry. We show for the first time that cUMP can be detected during embryogenesis and in adult organs of this vertebrate model system.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Uridina Monofosfato
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Peixe-Zebra
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CMP Cíclico
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Desenvolvimento Embrionário
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Nucleotídeos Cíclicos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article