Fluorescent Biotin Analogues for Microstructure Patterning and Selective Protein Immobilization.
Langmuir
; 31(46): 12573-8, 2015 Nov 24.
Article
em En
| MEDLINE
| ID: mdl-26559028
ABSTRACT
Benzyl substitution on ureido nitrogens of biotin led to manifestation of aggregation-induced emission, which was studied by steady-state fluorescence, microscopy, and TD-DFT, providing a rationale into the observed photophysical behavior. Besides exhibiting solvatochromism, the biotin derivatives revealed emission peaks centered at â¼430 and 545 nm, which has been attributed to the π-π stacking interactions. Our TD-DFT results also correlate the spectroscopic data and quantify the nature of transitions involved. The isothermal titration calorimetry data substantiates that the binding of the biotin derivatives with avidin are pretty strong. These derivatives on lithographic patterning present a platform for site specific strept(avidin) immobilization, thus opening avenues for potential applications exploiting these interactions. The fluorescent biotin derivatives can thus find applications in cellular biology and imaging.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Biotina
/
Proteínas Imobilizadas
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Microtecnologia
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Corantes Fluorescentes
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article