Development of a high yield expression and purification system for Domain I of Beta-2-glycoprotein I for the treatment of APS.
BMC Biotechnol
; 15: 104, 2015 Nov 14.
Article
em En
| MEDLINE
| ID: mdl-26576675
ABSTRACT
BACKGROUND:
In this paper we describe a novel method to achieve high yield bacterial expression of a small protein domain with considerable therapeutic potential; Domain I of Beta-2-glycoprotein I (ß2GPI). ß2GPI is intrinsic to the pathological progression of the Antiphospholipid Syndrome (APS). Patients develop autoantibodies targeting an epitope located on the N-terminal Domain I of ß2GPI rendering this domain of interest as a possible therapeutic.RESULTS:
This new method of production of Domain I of ß2GPI has increased the production yield by ~20 fold compared to previous methods in E.coli. This largely scalable, partially automated method produces 50-75 mg of pure, folded, active Domain I of ß2GPI per litre of expression media.CONCLUSION:
The application of this method may enable production of Domain I on sufficient scale to allow its use as a therapeutic.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes de Fusão
/
Síndrome Antifosfolipídica
/
Beta 2-Glicoproteína I
Limite:
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article