RNA Replication and Membrane Modification Require the Same Functions of Alphavirus Nonstructural Proteins.
J Virol
; 90(3): 1687-92, 2016 02 01.
Article
em En
| MEDLINE
| ID: mdl-26581991
ABSTRACT
The alphaviruses induce membrane invaginations known as spherules as their RNA replication sites. Here, we show that inactivation of any function (polymerase, helicase, protease, or membrane association) essential for RNA synthesis also prevents the generation of spherule structures in a Semliki Forest virus trans-replication system. Mutants capable of negative-strand synthesis, including those defective in RNA capping, gave rise to spherules. Recruitment of RNA to membranes in the absence of spherule formation was not detected.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vírus da Floresta de Semliki
/
Replicação Viral
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RNA Viral
/
Membrana Celular
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Proteínas não Estruturais Virais
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article