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miRNA-548p suppresses hepatitis B virus X protein associated hepatocellular carcinoma by downregulating oncoprotein hepatitis B x-interacting protein.
Hu, Xiu-Mei; Yan, Xiao-Hui; Hu, Yan-Wei; Huang, Jin-Lan; Cao, Shun-Wang; Ren, Ting-Yu; Tang, Yue-Ting; Lin, Li; Zheng, Lei; Wang, Qian.
Afiliação
  • Hu XM; Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Yan XH; Research Center of Clinical Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Hu YW; Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Huang JL; Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Cao SW; Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Ren TY; Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Tang YT; Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Lin L; Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Zheng L; Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Wang Q; Laboratory Medicine Center, Nanfang Hospital, Southern Medical University, Guangzhou, China.
Hepatol Res ; 46(8): 804-15, 2016 Jul.
Article em En | MEDLINE | ID: mdl-26583881
AIM: miR-548p is a recently identified and poorly characterized miRNA. However, its role of miR-548p in tumorigenesis and progression remains poorly understood. Here, we aimed to investigate the biofunction of miR-548p in hepatocellular carcinogenesis. METHODS: The expression levels of miR-548p were detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The role of miR-548p in hepatocellular carcinoma (HCC) was determined by colony formation, flow cytometry assay and nude mice xenograft experiments. miR-548p target genes were analyzed by miRNA target predication programs and verified by qRT-PCR, western blotting assay and dual-luciferase reporter assay. RESULTS: miR-548p is repressed by hepatitis B virus X protein (HBx) in HCC tumor tissues and hepatoma cells, and inhibited cell growth by inhibiting cell proliferation and promoting cell apoptosis. miR-548p directly downregulated the expression of hepatitis B x-interacting protein (HBXIP) by binding to the 3'-untranslated region of HBXIP mRNA. Further study showed that hepatocyte nuclear factor-4a (HNF4A) promoted the expression of miR-548p and inhibited the transcription of HBXIP. HNF4A is a dominant transcriptional regulator of hepatocyte differentiation and hepatocellular carcinogenesis, and is shown to be repressed by HBx. CONCLUSION: We proposed the model for HBx/HNF4A/miR-548p/HBXIP pathway that controls hepatoma cell growth and tumorigenesis of HCC. miR-548p was identified as a tumor-suppressor in HBx-associated hepatocellular carcinogenesis.
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Texto completo: 1 Base de dados: MEDLINE Tipo de estudo: Prognostic_studies / Risk_factors_studies Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Tipo de estudo: Prognostic_studies / Risk_factors_studies Idioma: En Ano de publicação: 2016 Tipo de documento: Article