Quantification of phosphatidylethanol 16:0/18:1, 18:1/18:1, and 16:0/16:0 in venous blood and venous and capillary dried blood spots from patients in alcohol withdrawal and control volunteers.
Anal Bioanal Chem
; 408(3): 825-38, 2016 Jan.
Article
em En
| MEDLINE
| ID: mdl-26597914
ABSTRACT
Phosphatidylethanol species (PEths) are promising biomarkers of alcohol consumption. Here, we report on the set-up, validation, and application of a novel UHPLC-ESI-MS/MS method for the quantification of PEth 160/181, PEth 181/181, and PEth 160/160 in whole blood (30 µL) and in venous (V, 30 µL) or capillary (C, 3 punches (3 mm)) dried blood spots (DBS). The methods were linear from 10 (LLOQ) to 2000 ng/mL for PEth 160/181, from 10 (LLOQ) to 1940 ng/mL for PEth 181/181, and from 19 (LLOQ) to 3872 ng/mL for PEth 160/160. Extraction efficiencies were higher than 55% (RSD < 18%) and matrix effects compensated for by IS were between 77 and 125% (RSD < 10%). Accuracy, repeatability, and intermediate precision fulfilled acceptance criteria (bias and RSD below 13%). Validity of the procedure for determination of PEth 160/181 in blood was demonstrated by the successful participation in a proficiency test. The quantification of PEths in C-DBS was not significantly influenced by the hematocrit, punch localization, or spot volume. The stability of PEths in V-DBS stored at room temperature was demonstrated up to 6 months. The method was applied to authentic samples (whole blood, V-DBS, and C-DBS) from 50 inpatients in alcohol withdrawal and 50 control volunteers. Applying a cut-off value to detect inpatients at 221 ng/mL for PEth 160/181 provided no false positive results and a good sensitivity (86%). Comparison of quantitative results (Bland-Altman plot, Passing-Bablok regression, and Wilcoxon signed rank test) revealed that V-DBS and C-DBS were valid alternatives to venous blood for the detection of alcohol consumption.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Síndrome de Abstinência a Substâncias
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Consumo de Bebidas Alcoólicas
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Cromatografia Líquida de Alta Pressão
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Glicerofosfolipídeos
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Espectrometria de Massas em Tandem
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Teste em Amostras de Sangue Seco
Tipo de estudo:
Evaluation_studies
Limite:
Adult
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Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article