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Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II.
Sierra, Raymond G; Gati, Cornelius; Laksmono, Hartawan; Dao, E Han; Gul, Sheraz; Fuller, Franklin; Kern, Jan; Chatterjee, Ruchira; Ibrahim, Mohamed; Brewster, Aaron S; Young, Iris D; Michels-Clark, Tara; Aquila, Andrew; Liang, Mengning; Hunter, Mark S; Koglin, Jason E; Boutet, Sébastien; Junco, Elia A; Hayes, Brandon; Bogan, Michael J; Hampton, Christina Y; Puglisi, Elisabetta V; Sauter, Nicholas K; Stan, Claudiu A; Zouni, Athina; Yano, Junko; Yachandra, Vittal K; Soltis, S Michael; Puglisi, Joseph D; DeMirci, Hasan.
Afiliação
  • Sierra RG; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Gati C; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Hamburg, Germany.
  • Laksmono H; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Dao EH; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Gul S; Lawrence Berkeley National Laboratory, Berkeley, California, USA.
  • Fuller F; Lawrence Berkeley National Laboratory, Berkeley, California, USA.
  • Kern J; Lawrence Berkeley National Laboratory, Berkeley, California, USA.
  • Chatterjee R; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Ibrahim M; Lawrence Berkeley National Laboratory, Berkeley, California, USA.
  • Brewster AS; Institute für Biologie, Humboldt University of Berlin, Berlin, Germany.
  • Young ID; Lawrence Berkeley National Laboratory, Berkeley, California, USA.
  • Michels-Clark T; Lawrence Berkeley National Laboratory, Berkeley, California, USA.
  • Aquila A; Lawrence Berkeley National Laboratory, Berkeley, California, USA.
  • Liang M; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Hunter MS; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Koglin JE; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Boutet S; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Junco EA; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Hayes B; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Bogan MJ; Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Hampton CY; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Puglisi EV; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Sauter NK; Department of Structural Biology, Stanford University School of Medicine, Stanford, California, USA.
  • Stan CA; Lawrence Berkeley National Laboratory, Berkeley, California, USA.
  • Zouni A; Stanford PULSE Institute, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • Yano J; Institute für Biologie, Humboldt University of Berlin, Berlin, Germany.
  • Yachandra VK; Lawrence Berkeley National Laboratory, Berkeley, California, USA.
  • Soltis SM; Lawrence Berkeley National Laboratory, Berkeley, California, USA.
  • Puglisi JD; Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, California, USA.
  • DeMirci H; Department of Structural Biology, Stanford University School of Medicine, Stanford, California, USA.
Nat Methods ; 13(1): 59-62, 2016 Jan.
Article em En | MEDLINE | ID: mdl-26619013
ABSTRACT
We describe a concentric-flow electrokinetic injector for efficiently delivering microcrystals for serial femtosecond X-ray crystallography analysis that enables studies of challenging biological systems in their unadulterated mother liquor. We used the injector to analyze microcrystals of Geobacillus stearothermophilus thermolysin (2.2-Å structure), Thermosynechococcus elongatus photosystem II (<3-Å diffraction) and Thermus thermophilus small ribosomal subunit bound to the antibiotic paromomycin at ambient temperature (3.4-Å structure).
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ribossomos / Cristalografia / Complexo de Proteína do Fotossistema II Idioma: En Ano de publicação: 2016 Tipo de documento: Article
Texto completo
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Ribossomos / Cristalografia / Complexo de Proteína do Fotossistema II Idioma: En Ano de publicação: 2016 Tipo de documento: Article