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Seed Dormancy in Arabidopsis Is Controlled by Alternative Polyadenylation of DOG1.
Cyrek, Malgorzata; Fedak, Halina; Ciesielski, Arkadiusz; Guo, Yanwu; Sliwa, Aleksandra; Brzezniak, Lien; Krzyczmonik, Katarzyna; Pietras, Zbigniew; Kaczanowski, Szymon; Liu, Fuquan; Swiezewski, Szymon.
Afiliação
  • Cyrek M; Institute of Biochemistry and Biophysics, Department of Protein Biosynthesis, Pawinskiego 5a, 02-106 Warsaw, Poland (M.C., H.F., A.C., Y.G., A.S., L.B., K.K., Z.P., S.K., S.S.); Warsaw University, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland (A.C.); andQueen's University Belfast, Schoo
  • Fedak H; Institute of Biochemistry and Biophysics, Department of Protein Biosynthesis, Pawinskiego 5a, 02-106 Warsaw, Poland (M.C., H.F., A.C., Y.G., A.S., L.B., K.K., Z.P., S.K., S.S.); Warsaw University, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland (A.C.); andQueen's University Belfast, Schoo
  • Ciesielski A; Institute of Biochemistry and Biophysics, Department of Protein Biosynthesis, Pawinskiego 5a, 02-106 Warsaw, Poland (M.C., H.F., A.C., Y.G., A.S., L.B., K.K., Z.P., S.K., S.S.); Warsaw University, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland (A.C.); andQueen's University Belfast, Schoo
  • Guo Y; Institute of Biochemistry and Biophysics, Department of Protein Biosynthesis, Pawinskiego 5a, 02-106 Warsaw, Poland (M.C., H.F., A.C., Y.G., A.S., L.B., K.K., Z.P., S.K., S.S.); Warsaw University, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland (A.C.); andQueen's University Belfast, Schoo
  • Sliwa A; Institute of Biochemistry and Biophysics, Department of Protein Biosynthesis, Pawinskiego 5a, 02-106 Warsaw, Poland (M.C., H.F., A.C., Y.G., A.S., L.B., K.K., Z.P., S.K., S.S.); Warsaw University, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland (A.C.); andQueen's University Belfast, Schoo
  • Brzezniak L; Institute of Biochemistry and Biophysics, Department of Protein Biosynthesis, Pawinskiego 5a, 02-106 Warsaw, Poland (M.C., H.F., A.C., Y.G., A.S., L.B., K.K., Z.P., S.K., S.S.); Warsaw University, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland (A.C.); andQueen's University Belfast, Schoo
  • Krzyczmonik K; Institute of Biochemistry and Biophysics, Department of Protein Biosynthesis, Pawinskiego 5a, 02-106 Warsaw, Poland (M.C., H.F., A.C., Y.G., A.S., L.B., K.K., Z.P., S.K., S.S.); Warsaw University, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland (A.C.); andQueen's University Belfast, Schoo
  • Pietras Z; Institute of Biochemistry and Biophysics, Department of Protein Biosynthesis, Pawinskiego 5a, 02-106 Warsaw, Poland (M.C., H.F., A.C., Y.G., A.S., L.B., K.K., Z.P., S.K., S.S.); Warsaw University, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland (A.C.); andQueen's University Belfast, Schoo
  • Kaczanowski S; Institute of Biochemistry and Biophysics, Department of Protein Biosynthesis, Pawinskiego 5a, 02-106 Warsaw, Poland (M.C., H.F., A.C., Y.G., A.S., L.B., K.K., Z.P., S.K., S.S.); Warsaw University, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland (A.C.); andQueen's University Belfast, Schoo
  • Liu F; Institute of Biochemistry and Biophysics, Department of Protein Biosynthesis, Pawinskiego 5a, 02-106 Warsaw, Poland (M.C., H.F., A.C., Y.G., A.S., L.B., K.K., Z.P., S.K., S.S.); Warsaw University, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland (A.C.); andQueen's University Belfast, Schoo
  • Swiezewski S; Institute of Biochemistry and Biophysics, Department of Protein Biosynthesis, Pawinskiego 5a, 02-106 Warsaw, Poland (M.C., H.F., A.C., Y.G., A.S., L.B., K.K., Z.P., S.K., S.S.); Warsaw University, Department of Chemistry, Pasteura 1, 02-093 Warsaw, Poland (A.C.); andQueen's University Belfast, Schoo
Plant Physiol ; 170(2): 947-55, 2016 Feb.
Article em En | MEDLINE | ID: mdl-26620523
ABSTRACT
DOG1 (Delay of Germination 1) is a key regulator of seed dormancy in Arabidopsis (Arabidopsis thaliana) and other plants. Interestingly, the C terminus of DOG1 is either absent or not conserved in many plant species. Here, we show that in Arabidopsis, DOG1 transcript is subject to alternative polyadenylation. In line with this, mutants in RNA 3' processing complex display weakened seed dormancy in parallel with defects in DOG1 proximal polyadenylation site selection, suggesting that the short DOG1 transcript is functional. This is corroborated by the finding that the proximally polyadenylated short DOG1 mRNA is translated in vivo and complements the dog1 mutant. In summary, our findings indicate that the short DOG1 protein isoform produced from the proximally polyadenylated DOG1 mRNA is a key player in the establishment of seed dormancy in Arabidopsis and characterizes a set of mutants in RNA 3' processing complex required for production of proximally polyadenylated functional DOG1 transcript.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sementes / Arabidopsis / Proteínas de Arabidopsis / Poliadenilação / Dormência de Plantas Idioma: En Ano de publicação: 2016 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sementes / Arabidopsis / Proteínas de Arabidopsis / Poliadenilação / Dormência de Plantas Idioma: En Ano de publicação: 2016 Tipo de documento: Article