Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology.
Microb Cell Fact
; 14: 196, 2015 Dec 02.
Article
em En
| MEDLINE
| ID: mdl-26627685
ABSTRACT
Promoters are indispensable elements of a standardized parts collection for synthetic biology. Regulated promoters of a wide variety of well-defined induction ratios and expression strengths are highly interesting for many applications. Exemplarily, we discuss the application of published genome scale transcriptomics data for the primary selection of methanol inducible promoters of the yeast Pichia pastoris (Komagataella sp.). Such a promoter collection can serve as an excellent toolbox for cell and metabolic engineering, and for gene expression to produce heterologous proteins.
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Base de dados:
MEDLINE
Assunto principal:
Pichia
/
Metanol
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Biologia Sintética
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article