Functional expression of xylose isomerase in flocculating industrial Saccharomyces cerevisiae strain for bioethanol production.
J Biosci Bioeng
; 121(6): 685-691, 2016 Jun.
Article
em En
| MEDLINE
| ID: mdl-26645659
ABSTRACT
Saccharomyces cerevisiae strains with xylose isomerase (XI) pathway were constructed using a flocculating industrial strain (YC-8) as the host. Both strains expressing wild-type xylA (coding XI) from the fungus Orpinomyces sp. and the bacterium Prevotella ruminicola, respectively, showed better growth ability and fermentation capacity when using xylose as the sole sugar than most of the reported strains expressing XI. Codon optimization of both XIs did not improve the xylose fermentation ability of the strains. Adaption significantly increased XI activity resulting in improved growth and fermentation. The strains expressing codon-optimized XI showed a higher increase in xylose consumption and ethanol production compared to strains expressing wild XI. Among all strains, the adapted strain YCPA2E expressing XI from P. ruminicola showed the best performance in the fermentation of xylose to ethanol. After 48 h of fermentation, YCPA2E assimilated 16.95 g/L xylose and produced 6.98 g/L ethanol. These results indicate that YC-8 is a suitable host strain for XI expression, especially for the codon-optimized XI originating from P. ruminicola.
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MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Reatores Biológicos
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Aldose-Cetose Isomerases
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Etanol
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Fermentação
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article