Inhibitory effect of withaferin A on Helicobacter pyloriinduced IL8 production and NFκB activation in gastric epithelial cells.
Mol Med Rep
; 13(1): 967-72, 2016 Jan.
Article
em En
| MEDLINE
| ID: mdl-26647855
ABSTRACT
Withaferin A (WA), a withanolide purified from Withania somnifera, has been known to exert anti-inflammatory effects. The present study sought to determine the effects of WA on Helicobacter (H.) pylori-mediated inflammation in the AGS gastric epithelial cell line. Cellular production of interleukin (IL)-8 and vascular endothelial growth factor (VEGF) was measured by ELISA. Western blot analysis was performed to determine the activation of nuclear factor (NF)-κB and mitogen-activated protein kinases (MAPKs) as well as hypoxia-inducible factor 1α stabilization. Bacterial growth was also examined by measuring the optical density. Pre-treatment or co-treatment with WA efficiently reduced IL-8 production by AGS cells in response to H. pylori infection. H. pylori-induced activation of NF-κB, but not MAPKs, was also inhibited by pre-treatment of WA in the cells. However, WA did not affect VEGF production and HIF-1α stabilization induced by H. pylori in AGS cells. In addition, WA did not influence the growth of H. pylori, suggesting that the anti-inflammatory effect of WA was not due to any bactericidal effect. These findings indicate that WA is a potential preventive or therapeutic agent for H. pylori-mediated gastric inflammation.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Interleucina-8
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Helicobacter pylori
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Infecções por Helicobacter
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Vitanolídeos
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Inflamação
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article