[QTRAP LC-MS/MS Analytical Study on Nucleosides and Nucleobases of Pseudostellariae Radix Cultivated in Different Idioplasm Resources].

ABSTRACT

OBJECTIVE: To analyze nucleosides and nucleobases of Pseudostellariae Radix cultivated in different idibplasni resources and to compare the differences. METHODS: QTRAP LC-MS/MS method was applied for the analysis of 13 kinds of nucleosides and nucleobases in Pseudostellariae Radix and the data obtained was analyzed by SPSS 16. 0 software. RESULTS: There were some differences between Pseudostellariae Radix cultivated in different idioplasm resources. The highest amount of nucleosides and nucleobases was ZS2 which came from Zherong in Fujian Province. The total content of nucleosides and nucleobases in the sample from Shibing in Guizhou Province was the lowest. There was little difference between ZS1 (Zherong in Fujian Province) and XC(Xuancheng in Anhui Province). CONCLUSION: This study provides evidence for the influence of eco-environment on the metabolites of Pseudostellariae Radix.