A Universal Polymerase Chain Reaction Developer.
Angew Chem Int Ed Engl
; 55(6): 2157-60, 2016 Feb 05.
Article
em En
| MEDLINE
| ID: mdl-26676946
ABSTRACT
The versatility of PCR, the gold standard for amplification of DNA targets, is hampered by the laborious, multi-step detection based on gel electrophoresis. We propose a one-step, one-tube method for the rapid (5â
min) naked-eye detection of PCR products, based on controlled aggregation of gold nanoparticles. Our method is universal, instrument-free, and ultra-sensitive, as it could detect as low as 0.01 zeptomoles of HIV template DNA in an excess of interfering human genomic DNA.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Reação em Cadeia da Polimerase
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Nanopartículas Metálicas
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Ouro
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article