MdSOS2L1 phosphorylates MdVHA-B1 to modulate malate accumulation in response to salinity in apple.
Plant Cell Rep
; 35(3): 705-18, 2016 Mar.
Article
em En
| MEDLINE
| ID: mdl-26687966
ABSTRACT
KEY MESSAGE Salt-induced phosphorylation of MdVHA-B1 protein was mediated by MdSOS2L1 protein kinase, and thereby increasing malate content in apple. Salinity is an important environmental factor that influences malate accumulation in apple. However, the molecular mechanism by which salinity regulates this process is poorly understood. In this work, we found that MdSOS2L1, a novel AtSOS2-LIKE protein kinase, interacts with V-ATPase subunit MdVHA-B1. Furthermore, MdSOS2L1 directly phosphorylates MdVHA-B1 at Ser(396) site to modulate malate accumulation in response to salt stress. Meanwhile, a series of transgenic analyses in apple calli showed that the MdSOS2L1-MdVHAB1 pathway was involved in the regulation of malate accumulation. Finally, a viral vector-based transformation approach demonstrated that the MdSOS2L1-MdVHAB1 pathway also modulated malate accumulation in apple fruits with or without salt stress. Collectively, our findings provide a new insight into the mechanism by which MdSOS2L1 phosphorylates MdVHA-B1 to modulate malate accumulation in response to salinity in apple.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
/
Proteínas Serina-Treonina Quinases
/
Malus
/
ATPases Vacuolares Próton-Translocadoras
/
Proteínas de Arabidopsis
/
Salinidade
/
Malatos
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article