Molecular screening for Rickettsia, Anaplasmataceae and Coxiella burnetii in Rhipicephalus sanguineus ticks from Malaysia.
Trop Biomed
; 32(2): 390-8, 2015 Jun.
Article
em En
| MEDLINE
| ID: mdl-26691268
ABSTRACT
A total of 44 Rhipicephalus sanguineus ticks collected from 23 dogs from Malaysia were screened for Rickettsia, Anaplasmataceae and Coxiella burnetii. Coxiella burnetii was detected in 59% (26/44) of ticks however Rickettsia and Anaplasmataceae were not detected in any of the ticks. In order to genotype the strains of C. burnetii, multispacer sequence typing (MST) was carried out using three different spacers. One of the spacers; Cox2 successfully amplified a fragment for which the full length sequence of 397 bp was obtained. The sequenced product revealed only a single nucleotide difference with the Cox2.3 type sequence.
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Base de dados:
MEDLINE
Assunto principal:
Rickettsia
/
Coxiella burnetii
/
Rhipicephalus sanguineus
/
Anaplasmataceae
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Animals
País como assunto:
Asia
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article