Two-Dimensional Crystallization of the Ca(2+)-ATPase for Electron Crystallography.
Methods Mol Biol
; 1377: 421-41, 2016.
Article
em En
| MEDLINE
| ID: mdl-26695053
Electron crystallography of two-dimensional crystalline arrays is a powerful alternative for the structure determination of membrane proteins. The advantages offered by this technique include a native membrane environment and the ability to closely correlate function and dynamics with crystalline preparations and structural data. Herein, we provide a detailed protocol for the reconstitution and two-dimensional crystallization of the sarcoplasmic reticulum calcium pump (also known as Ca(2+)-ATPase or SERCA) and its regulatory subunits phospholamban and sarcolipin.
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MEDLINE
Assunto principal:
Microscopia Eletrônica
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Cristalização
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ATPases Transportadoras de Cálcio do Retículo Sarcoplasmático
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article