Variability of ß-lactam susceptibility testing for Streptococcus pneumoniae using 4 commercial test methods and broth microdilution.
Diagn Microbiol Infect Dis
; 84(3): 240-5, 2016 Mar.
Article
em En
| MEDLINE
| ID: mdl-26707068
ABSTRACT
Limited data are available that verify the performance of commercial susceptibility methods for Streptococcus pneumoniae following the 2008 Clinical and Laboratory Standards Institute revision of the ß-lactam breakpoints. We compared the performance of Etest, M.I.C. Evaluator (M.I.C.E), Vitek, and Sensititre systems to broth microdilution for S. pneumoniae susceptibility testing of penicillin, ceftriaxone, meropenem, and amoxicillin. Essential agreement was ≥90% for the majority of the ß-lactams and methods tested, particularly for penicillin and ceftriaxone. Categorical agreements (CAs) for penicillin using meningeal and nonmeningeal breakpoints were ≥90%; CAs using penicillin oral breakpoints were 84-89%. Ceftriaxone CAs using nonmeningeal and meningeal breakpoints were 68-88% for Etest, M.I.C.E., and Vitek2 with 6-12% very major errors (VMEs) using meningeal breakpoints. Sensititre CAs for ceftriaxone, amoxicillin, and meropenem were ≥90% with no VMEs. In the context of the current guidelines, there exists considerable method-dependent variability in the susceptibility of S. pneumoniae to ß-lactams.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Kit de Reagentes para Diagnóstico
/
Streptococcus pneumoniae
/
Testes de Sensibilidade Microbiana
/
Beta-Lactamas
/
Antibacterianos
Tipo de estudo:
Diagnostic_studies
/
Guideline
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article